General Cell Based Assays
Meso Scale Discovery U-PLEX Mouse IL-10 ASY
The Meso Scale Discovery U-PLEX Mouse IL-10 Assay is an electrochemiluminescent immunoassay for the quantitative measurement of mouse interleukin 10 (IL-10) in serum plasma cell culture supernatants and other biological samples This assay utilizes MSD s proprietary SECTOR Imager and provides high sensitivity a broad dynamic range and requires low sample volume The lower limit of detection is 0 04 pg/mL This assay is for research use only and is not intended for diagnostic procedures The kit includes a 96-well plate coated with capture antibody detection antibody calibrator and assay diluent
Eurofins Discoverx PathHunter eXpress TACR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0344E2CP0L)
The PathHunter TACR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. substance P) based activation of TACR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged TACR1 and EA-tagged β-Arrestin-2. Activation of TACR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
TRIBIOSCIENCE INC Glycerol Colorimetric Assay 100 Tests
Tribioscience’s Glycerol Colorimetric Assay kit is a robust, simple, and high throughput method based on coupled enzyme reactions. Glycerol is phosphorylated and oxidized to generate hydrogen peroxide, which is catalyzed and reacts with the probe measured by the colorimetric method at OD = 570 nm.
Eurofins Discoverx PathHunter eXpress mINSRb Functional Assay, 2-Plate Kit. (93-1017E3CP16M)
The PathHunter eXpress mINSRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated mINSRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged mINSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Biolegend APC/FireTM 750 anti-human MERT
APC/Fire™ 750 anti-human MERTK [590H11G1E3]; Isotype: Mouse IgG1, κ; Reactivity: Human; Apps: FC; Size: 100 tests
Sigma Aldrich Fine Chemicals Biosciences fluorometric Intracellular
Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels ROS contribute to cell signaling and host defense. Increased ROS generation above the detoxification capacity of the biological system results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids essential proteins and DNA. ROS has been implicated in disease states such as Alzheimers disease Parkinsons disease cancer and aging.
Eurofins Discoverx PathHunter eXpress CHRM2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0198E2CP0L)
The PathHunter CHRM2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. oxotremorine) based activation of CHRM2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM2 and EA-tagged β-Arrestin-2. Activation of CHRM2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 23 Reagent, 500. (93-0563R23B)
AssayComplete Cell Plating 23 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx cAMP Hunter eXpress ADRA2C CHO-K1 GPCR Assay, 2-Plate Kit. (95-0053E2CP2M)
The cAMP Hunter ADRA2C eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2C GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRA2C, a naturally Gi/Go coupled GPCR. Upon activation of ADRA2C, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human EGFR ASY
The R-PLEX Human EGFR Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay measuring total EGFR levels in human serum plasma and cell culture supernatants This electrochemiluminescence (ECL) assay uses MSD s SULFO-TAG labels and multi-array plate technology for simultaneous detection of multiple protein biomarkers from a single sample It offers high sensitivity broad dynamic range low sample volume and rapid results The kit includes a 96-well plate with capture antibody detection antibody calibrator and assay diluent suitable for biomarker discovery disease research and drug development
Eurofins Discoverx PathHunter eXpress CSF1R/CSF1R Dimerization Assay, 2pl Kit. (93-1061E3CP5M)
The PathHunter CSF1R/CSF1R Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. M-CSF) induced dimerization of CSF1R/CSF1R receptors. This product uses EFC technology. The cell line included overexpresses CSF1R target protein fused to PK alongside CSF1R target protein fused to EA. Upon ligand-induced dimerization of CSF1R/CSF1R, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Teriparatide Bioassay Kit, 10pl. (95-0118Y2-00058)
The cAMP Hunter Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress GPR123 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0417E2ACP1M)
The PathHunter GPR123 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR123 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR123 and EA-tagged β-Arrestin-2. Activation of GPR123 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Molecular Devices FLIPR Calcium 6-QF Assay Kit, Quench-free, 10 plates
The FLIPR Calcium 6-QF Assay Kit (Explorer kit) is a quench-free, mix-and-read fluorescent assay for measuring intracellular calcium flux in high-throughput plate formats. The Calcium 6-QF formulation provides an increased signal window and is suitable for quench-sensitive targets and multiplexed assays.
- Quench-free formulation for quench-sensitive targets and multiplexing.
- Designed for mix-and-read workflows with pre-optimized FLIPR and FlexStation protocols.
- Sufficient reagents for 10 plates in Explorer kit format.
- Each reagent vial for Sample and Explorer kits is sufficient for one 96-, 384-, or 1536-well plate.
- Suitable for endogenous, primary, and stem cell targets with improved signal window and assay robustness.